A functional analysis of T-DNA gene 6b: The fine tuning of ...

The physiological function in planta of T-DNA gene 6b was studied under various experimental conditions. For this purpose the coding region of gene 6b was cloned behind the 1′-promoter of the TR-DNA to enhance expression of the gene product in transformed plant cells. Expression of the recombinant gene in leaf discs of Nicotiana tabacum altered the capacity for shoot formation of the …

CTX-M and Plasmid-mediated AmpC-Producing ...

Data from the Guyana sugar planta-tions must include factors other than DDT and malaria because profound differences existed in all-cause mor-tality in adults and children over the span of the reports. Whether DDT was effective in those areas at that time cannot determine whether it would be a two-edged sword now. Although the p,p′ isomer of DDE

Highly conserved protein kinases involved in the ...

2004-1-1 · Activation of AMPK by AMP is antagonized by high (mM) concentrations of ATP and a high AMP:ATP ratio is symptomatic of low cellular energy levels. SNF1 activity responds sensitively to glucose levels, but the exact mechanisms involved in sensing glucose levels and initiating a signal through the SNF1 pathway are not understood.

Screening of Transient Receptor Potential Canonical ...

2016-3-1 · Transient receptor potential canonical (TRPC) proteins form Ca2+-permeable cation channels activated upon stimulation of metabotropic receptors coupled to phospholipase C. Among the TRPC subfamily, TRPC3 and TRPC6 channels activated …

(PDF) Screening for phosphodiesterase inhibitory activity ...

Bioorganic & Medicinal Chemistry Letters 12, 2313–2316. Sittiwicheanwong, P., 1999. Screening for biologically active compounds from gies of plant selection could successfully lead to sources of PDEs medicinal plants by inhibition of cyclic-AMP phosphodiesterase. Master Thesis, inhibitors.

Insulin Plant: Does It Cure Diabetes? Benefits, Dosage ...

2019-1-30 · Insulin plant was introduced to India in recent times. The plant has been regarded as being a magical, natural cure for diabetes. Even though the herb is primarily used for curing diabetes, it is also beneficial in the treatment of kidney stones, blood …

Isolation of antimicrobial peptides from different plant ...

2020-10-23 · Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolation of AMPs from plant organs based on the type of extractant and initial ...

Control of inflorescence architecture in tomato by …

SlBOPs are recruited to the nucleus by TMF to enhance its transcriptional activity. We showed previously that TMF, like other ALOG proteins, is a weak transcriptional activator (MacAlister et al. 2012), and, consistent with this, a recent comparative bioinformatics analysis of ALOG proteins revealed a DNA-binding domain with distant homology with retrotransposon integrase proteins (Fig. 2A ...

Screening a cDNA Library for Protein–Protein Interactions ...

Abstract. Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners.

Development of "Purple Endosperm Rice" by Engineering ...

2017-7-5 · The LB-agar plates for colony screening were supplemented with Kan (25 μg/ml) and Amp (70 μg/ml). The plasmid mixture was prepared with hundreds of pooled colonies, as above. About 50–100 ng of the plasmid DNA was digested with 1 unit (1 μl) of homing endonuclease PI- Sce I in a 10-μl reaction at 37°C for 1–2 h to select the target ...

Evaluation of methods for plant genomic DNA sequence ...

Genomic DNA was eluted with 100 μL of elution buffer in the kit. Then, 1 μL of solution containing 75.5–116 ng of N. benthamiana genomic DNA or 6.85–9.75 ng of A. thaliana genomic DNA was used for PCR. PCR was performed in a 20 μL reaction solution of KOD FX Neo (Toyobo) containing 1 μL of the sample solution and 4 pmol of each primer.

An Efficient Agrobacterium‐Mediated Transformation ...

2021-3-3 · Screening. Successful transformation should be confirmed by PCR amplification of the hygromycin resistance gene and/or copy number analysis performed by quantitative PCR. DNA extraction. 11. Harvest 0.5- to 0.7-cm leaf samples into PCR tubes, and extract DNA with Extract-N-Amp™ Plant Tissue PCR Kits following the manufacturer''s instructions.

A cysteine-rich antimicrobial peptide from Pinus monticola ...

2012-2-21 · Canola (Brassica napus), an agriculturally important oilseed crop, can be significantly affected by diseases such as sclerotinia stem rot, blackleg, and alternaria black spot resulting in significant loss of crop productivity and quality. Cysteine-rich antimicrobial peptides isolated from plants have emerged as a potential resource for protection of plants against phytopathogens. Here we ...

Proximity-dependent biotinylation screening identifies ...

2019-7-19 · Lin Q, Zhou Z, Luo W, Fang M, Li M, Li H. Screening of proximal and interacting proteins in Rice protoplasts by proximity-dependent Biotinylation. Front Plant Sci. 2017;8(10):749. Article Google Scholar 17. Khan M, Youn J-Y, Gingras A-C, Subramaniam R, Desveaux D. In planta proximity dependent biotin identification (BioID). Sci Rep. 2018;8(1):9212.

Thieme E-Journals

Introduction. Ginseng Radix (Panax ginseng C. A. Meyer, Araliaceae) is one of the most widely used medicinal herbs in the world, particularly in the Far East, China, Japan, and Korea.The most frequently used part of this plant for traditional therapeutic applications is the root (rhizome and/or radix).

Development of "Purple Endosperm Rice" by Engineering ...

2017-6-27 · The LB-agar plates for colony screening were supplemented with Kan (25 μg/ml) and Amp (70 μg/ml). The plasmid mixture was prepared with hundreds of pooled colonies, as above. About 50–100 ng of the plasmid DNA was digested with 1 unit (1 μl) of homing endonuclease PI- Sce I in a 10-μl reaction at 37°C for 1–2 h to select the target ...

(PDF) Natural and Herbal Remedies for Cancer Treatment

2016-10-15 · Thus, screening phytochemicals as anticancer agents could be a better approach in cancer prevention and treatment. This chemoprevention approach may have translational significance for prevention ...

Enzyme‐driven metabolomic screening: a …

2016-6-29 · The reaction was left for 4 h at room temperature and stopped by the addition of methanol to a final concentration of 50%. Standards for retention time alignment (caffeine 0.4 ng μl −1 and reserpine 0.5 ng μl −1) were added to the samples and then centrifuged at 20 000 g for 10 min to pellet any precipitate. The supernatant was removed ...

Using CRISPR/ttLbCas12a for in planta Gene Targeting in A ...

2020-8-31 · Outline of the in planta gene targeting system (Fauser et al., 2012).The ipGT system is based on the DSB induction within the target locus and the excision of the HR donor sequence. The HR donor contains the desired mutation, which should be integrated in the target locus and silent mutations to avoid cleavage within the donor molecule.

Sheath blight of rice: a review and ...

2019-7-25 · Main conclusion Rice sheath blight research should prioritise optimising biological control approaches, identification of resistance gene mechanisms and application in genetic improvement and smart farming for early disease detection. Abstract Rice sheath blight, caused by Rhizoctonia solani AG1-1A, is one of the most devasting diseases of the crop. To move forward with effective crop ...

Plasticity of rosette size in response to nitrogen ...

2021-7-6 · 3.1 Plasticity screening using Arabidopsis thaliana accessions in response to N availability We screened 190 Arabidopsis accessions (Table S1 ), grown on soil supplemented with three different concentrations of N (Methods), for plasticity of focal traits including early rosette diameter (ERD), final rosette diameter (FRD), flowering time (FT ...

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SPIE Homepage. Celebrate this annual, global initiative that provides a focal point for the continued appreciation of light. Join colleagues online. You can still register and secure extended access to latest research. Join industry leaders for two days of in-person exhibitions. Hear latest research shared over three days of technical programming.

Screening for phosphodiesterase inhibitory activity of ...

2008-9-26 · Nineteen plants used as aphrodisiac and neurotonic agents in Thai traditional medicines and twelve Leguminosae plants were collected. The ethanolic extracts of these plants were screened for their PDE inhibitory activity using the radioassay, SPA, in 96 …

A TaqMan-Based Multiplex qPCR Assay and DNA …

2015-10-1 · Ralstonia solanacearum race 3 biovar 2 strains belonging to phylotype IIB, sequevars 1 and 2 (IIB-1&2) cause brown rot of potato in temperate climates, and are quarantined pathogens in Canada and Europe. Since these strains are not established in the U.S. and because of their potential risk to the potato industry, the U.S. government has listed them as select agents.

A blueprint for gene function analysis through Base ...

2021-1-9 · One possible way to modify a gene in planta is the TILLING strategy, based on EMS-induced mutations in the genome (Jacob et al., 2018). However, such in vivo mutagenesis is not targeted to a specific locus and, for this reason, needs first a thorough selection system to …

Microbial Interkingdom Interactions in Roots Promote ...

2018-11-1 · The concentration of DNA samples was fluorescently quantified, diluted to 3.5 ng/µL, and used in a two-step PCR amplification protocol. In the first step, V4–V7 of bacterial 16S rRNA (799F - 1192R), fungal ITS1 (ITS1F - ITS2) and oomycetal ITS1 (ITS1-O - 5.8 s-Rev-O) were amplified. Under a sterile hood, each sample was amplified in ...

Identification and molecular characterization of the ...

2013-7-29 · Amp R was used as a selection marker for screening of E. coli clones harboring the pBC1 plasmid. E. coli was grown in 1 l of Luria Bertani (LB) broth containing 1% tryptone, 0.5% of yeast extract, 1% NaCl and ampicillin (final concentration of ampicillin:100 µg/ml).

A Novel mRNA Level Subtraction Method for Quick ...

2009-12-24 · Subtraction technique has been broadly applied for target gene discovery. However, most current protocols apply relative differential subtraction and result in great amount clone mixtures of unique and differentially expressed genes. This makes it more difficult to identify unique or target-orientated expressed genes. In this study, we developed a novel method for subtraction at mRNA level by ...

Proximity-dependent biotinylation screening identifies ...

2019-7-19 · Autophagy is a conserved, highly-regulated catabolic process that plays important roles in growth, development and innate immunity in plants. In this study, we compared the rate of autophagy induction in Nicotiana benthamiana plants infected with Tobacco mosaic virus or the TMV 24A + UPD mutant variant, which replicates at a faster rate and induces more severe symptoms.

Overexpression of nicotinamidase 3 (NIC3) gene and the ...

2021-8-30 · Key message Overexpressing Nicotinamidase 3 gene, and the exogenous application of its metabolite nicotinic acid (NA), enhance drought stress tolerance and increase biomass in Arabidopsis thaliana. Abstract With progressive global climatic changes, plant productivity is threatened severely by drought stress. Deciphering the molecular mechanisms regarding genes responsible for balancing …

Identification and characterization of the first cytokinin ...

2019-3-29 · Cytokinins are one of the five major hormones families in plants and are important for their normal growth and environmental adaptability. In plants, cytokinins are mostly present as glycosides in plants, and their glycosylation modifications are catalyzed by family 1 glycosyltransferases. Current research on cytokinin glycosylation has focused on the biochemical identification of enzymes and ...

(PDF) Phytochemical and antimicrobial studies on ...

Fitoterapia 72 Ž2001. 54᎐56 Short report Phytochemical and antimicrobial studies on Butyrospermum paradoxum Isiaka A. Ogunwande a, Muibat O. Bello a, Olayide N. Olawore a,U, Kamaldin A. Muili b a Department of Pure and Applied Chemistry, Ladoke Akintola Uni¨ ersity of Technology, P.M.B. 4000, Ogbomoso, Nigeria b Department of Medical Microbiology, Uni¨ ersity College Hospital, (U.C.H ...

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